Volume 10, Issue 5 (September & October 2019)                   BCN 2019, 10(5): 7-7 | Back to browse issues page


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Grüßer L, Blaumeiser-Debarry R, Rossaint R, Krings M, Kremer B, Höllig A et al . A Six-step Approach to Gain Higher Quality Results From ‎Organotypic Hippocampal Brain Slices in a Traumatic Brain ‎Injury Model. BCN. 2019; 10 (5) :7-7
URL: http://bcn.iums.ac.ir/article-1-1019-en.html
1- Department of Anesthesiology, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
2- Department of Anesthesiology and Intensive Care, Medizinisches Zentrum StaedteRegion Aachen, Mauerfeldchen 25, 52146 Wuerselen, Germany
3- Department of Neurosurgery, RWTH Aachen University Hospital, Pauwelsstr. 30, 52074 Aachen, Germany
Abstract:  
Background: Organotypic Hippocampal Brain Slices (OHBS) provide a better alternative to in vivo models to scrutinize Traumatic Brain Injury (TBI). We followed a well-established TBI protocol but noticed that several factors might influence the results in such a set-up. Here, we describe a structured approach to generate more comparable results and discuss why specific eligibility criteria should be applied.
Methods: We defined necessary checkpoints and developed inclusion and exclusion criteria that took into consideration the observed variation in such a model. Objective measures include the identification and exclusion of pre-damaged slices and outliers. In this study, six steps were outlined.
Results: A six-step approach to enhance comparability is proposed and summarized in a flowchart. We applied the suggested measures to data derived from our TBI-experiments, examining the impact of three different interventions in 1459 OHBS. Our exemplary results show that more precise findings are ensured through equal requirements set for all slices.
Conclusion: Results in a TBI experiment on OHBS should be critically analyzed as inhomogeneity may occur. In other words, a structured approach to comparing the results should be followed to ensure achieving more precise findings. Further research is recommended to confirm and further develop this framework.
     
Type of Study: Original | Subject: Cellular and molecular Neuroscience
Received: 2017/09/7 | Accepted: 2018/09/22

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