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Showing 17 results for ESLAMI

Iraj Nazery, Massoud ESLAMI,
Volume 6, Issue 1 (5-1992)

Brucella endocarditis is an uncommon but not rare manifestation of brucellosis in our country. We report six adult patients aged 20 to 45 years. Two of our patients were female, and this is the first time that Brucella endocarditis was reported in adult females. In five cases, aortic valve replacement was performed in addition to combined antibiotic therapy. One of them died due to immediate complication of cardiac surgery, but the others had an excellent recovery. Our study shows that medical treatment is not sufficient for Brucella mellitensis endocarditis, and urgent cardiac valve replacement must be considered. The prognosis will not be poor with combined medical and surgical therapy.
A Farhoudl, Mb ESLAMI, A Shakurnia,
Volume 7, Issue 4 (2-1994)

Serum IgG subclass levels were measured using an indirect immunoenzymatic assay (ELISA) with monoclonal antibodies in 16 children with asthma and 13 children with atopy who had mostly recurrent infections. Seven of the asthmatic children had marked low or low normal levels of IgG4, six had marked low or low normal levels of IgG 3, two had marked low normal levels of both IgG2, and IgG 3 and one had low levels of IgG2 " IgG3, and IgG4 •. All these patients suffered from recurrent sinopulmonary infections. There were low percentages of IgG3, and IgG4. defects (about 15%) in the atopic patients, while a significant increase in the serum IgG4. levels were observed (six patient out of 13 patients, 46.2%).
I I. nazery, A Ghaemian, M ESLAMI,
Volume 9, Issue 3 (10-1995)

Between I 9H I and 1993,30 cases of primary cardiac tumors were diagnosed at the Imam Khomeini Hospital. 20 patients were female and 10 were male, with an average age of 32 years (ranging from 17 days to 65 years of age). The mean duration of symptoms before diagnosis was 6 months, with dyspnea and palpitation being the most common symptoms (60%) and cardiac' murmurs the most usual signs (X4%). The diagnosis of cardiac tumors was made by echocardiography in all patients. Transesophageal echocardiography and cardiac catheterization were performed in X and LJ patients respectively for more precise localization of the tumor and evaluation of the coronary arteries. The left atrium was the only site of tumor in 60%. 4 patients had tumors in more than one chamber. 27 patients underwent surgery without mortality while J patients did not undergo surgery. Pathological examination revealed benign myxoma in 23 patients, fibroma (1 patient), round cell sarcoma (1 patient), chondrosarcoma (1 patient), liposarcoma (1 patient), and undifferentiated sarcoma (1 patient).
Aa Pourfathollah, Mb ESLAMI, Ar Yalda, Af Sarraf Nejat,
Volume 10, Issue 3 (11-1996)

Human brucellosis is a major health problem in developing countries. A number of patients with human brucellosis do not recover from the acute stage of the disease and pass into the chronic form. Since the pathophysiology of this change is not well understood, we studied the T-cell subsets during the acute, subacute and chronic forms of human brucellosis. In this study we found alterations in T-Iymphocyte subsets and in the CD4/CD8 ratio in subacute and chronic brucellosis while no significant change was found in T-cell subsets in acute brucellosis. We believe that this alteration of T-cell subsets is important in the pathophysiology of brucellosis, but it is not clear whether brucella cause this change or a primary immunodysfunction in patients with a disturbance in T-cell subset regulation prevents recovery from acute brucellosis and leads to the development of chronic brucellosis.
Mh Roustai, R Nategh, M Noroozi, Mb ESLAMI, M Mahmoodi, T Mokhtari Azad, K Holakooe Naeeni, M Pezeshki,
Volume 10, Issue 3 (11-1996)

A micro neutralization test was used for the detection and evaluation of mumps neutralizing antibodies. A total of 1037 blood samples from selected groups of students aged 7 to 22 years in south and east of Tehran, 139 blood samples from the umbilical cords of pregnant women at two Tehran hospitals at delivery time, and 190 samples from children under five years of age who were living in two villages located in Zandjan province, were tested. The results of this study indicated that the percentage of the above mentioned sera having mumps virus neutralizing antibodies with a titer of 1:2 or greater was 69.6%,92.8%, and 29.5%, respectively. Vaccination against mumps is optional in the Islamic Republic of Iran and therefore it is believed that the virus is circulating among susceptible subjects. These data also suggest that children could be a target for mumps vaccination.
A Zavaran Hosseini, Mb ESLAMI, M Jalali,
Volume 10, Issue 4 (2-1997)

Mycobacterium tuberculosis (H37Rv strain) was used in this study. The bacterial cells were disintegrated by sonication. The separation and characterization of the soluble molecules were attempted by various techniques including gel filtration, ion exchange chromatographies and polyacrylamide gel electrophoresis, using SDS and 2ME. Eight protein molecules with molecular weights ranging from 6.3 up to 204 kD were identified. Following reduction of the 204 kD molecule with 2ME, six smaller molecules with 12a, 12b, 21, 29,45 and 81.5 kD molecular weights were obtained. All isolated protein molecules were able to induce delayed hypersensitivity skin reaction in sensitized guinea pigs and proliferation of T-cells in vitro. Regarding the fact that an effective protective immunity in tuberculosis is dependent mainly on T-cell response, it is suggested that the molecules isolated in this study may be useful in conceiving a vaccine and/or diagnostic tests for tuberculosis.
Fereshteh Shahcheraghi, Mohammad Bagher ESLAMI, Bahman Tabaraie, Ghorban Behzadian-Nejad,
Volume 12, Issue 1 (5-1998)

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminants. The OMP preparation was purified by column chromatography on Sephacryl S-200. Three major symmetrical peaks emerged from the column with kav values of 1. 81, 2.42 and 2.56 in succession in addition to a few closely related minor peaks. Characterization of crude OMP on SDS-PAGE showed 13 protein bands. The three major peaks 1,2 and 3 were subjected to SDS-PAGE separately and the molecular weights of peaks 2 and 3 were calculated to be 26 and 38 kDa, respectively and the first peak was further resolved into two subfractions with molecular weights of 62 and 67 kDa. However, after treatment of OMP with trypsin the number of bands were reduced to one prominent band with a molecular weight of 38 kDa and a thinner band of 41 kDa.
K Ahmadi, Mb ESLAMI,
Volume 12, Issue 4 (2-1999)

Neonatal tetanus (NT) is a leading cause of neonatal mortality in many parts of the world. Tetanus is a vaccine-preventable disease and is second only to measles worldwide as a cause of childhood mortality. In this study the various irnrnunoglobulin classes of anti-tetanus antibody in the sera of 105 Iranian women of childbearing age (13-45 years) were titrated by enzyme linked immunosorbent assay (ELISA). Our results demonstrate that the majority of women (96.29%) had protective anti-tetanus toxoid antibody. All women were negative for 19E. 92.39%, 91.43% and 80.96% of women were found to be positive for IgG, IgA and IgM, respectively. Therefore, with respect to lack of protective anti-tetanus antibody in a minority of women, our results suggest that this group might be at risk of tetanus disease and if they get pregnant, need to be vaccinated against tetanus in order to produce sufficient Ab for their own protection and to provide their fetuses with anti-tetanus antibody.
H Goudarzi, B Kazemi, F Falah, G ESLAMI, M Ghazi, F Doustdar,
Volume 17, Issue 2 (8-2003)

Tuberculosis remains as an important socioeconomical and medical problem throughout the world and especially in Iran. Early and timely diagnosis of pulmonary and extrapulmonary tuberculosis is vital to initiate prompt treatment. Current diagnostic methods are either slow or lack enough sensitivity or specificity. Several mycobacterial antigens are involved in the complex interaction with the immune system of the host. Their identification is important for both diagnosis and protection against mycobacteria. Antigen 60 (A60) is a thermostable antigen found in the cytosol of M. bovis and M. tuberculosis. An ELISA test using A60 is designed for diagnosis of tuberculosis with satisfactory results. In previous studies, A60 has also showed a protective effect against experimental infections and useful immunotherapeutic effects in promotion of cancer development. In the present work we tried to purify A60 from the cytoplasm of BCG. A60 was purified by exclusion gel chromatography using sepharose 4B. A60 was recognized by bidimensional immunoelectrophoresis with anti-BCG and anti-A60 antiserum, where it appears as the less mobile component. In agarose electrophoresis, A60 showed only one band but in immunodiffusion it showed two immunoprecipitinogen lines with anti-BCG anti-serum. In analyzing with dot blotting, both cytoplasm and cell wall of BCG showed positive reaction with antiA60 anti-serum. When A60 was fractionated by SDS-PAGE and analyzed by: western blot using anti-A60 antibody, 65,46, 40, 38 and 35 KDa protein fractions' were identified. It is concluded that A60 is a macromolecular antigen of BCG with a molecular weight of 106_107 Da and is a lipoprotein-polysaccharide complex which contains several proteins. A60 is present in both cytoplasm and cell wall of BCG and can easily be purified from BCG vaccine using exclusion chromatography by sepharose 4B, to be used for designing diagnostic tests for TB.
Zahra Asgari, Foroogh Bahreini, Haydeh Samiee, Bita ESLAMI, Afsaneh Tehranian, Somayeh Sabet,
Volume 22, Issue 1 (5-2008)


  Background: Hysterectomy is the second most common major surgery procedure

  done after cesarean section by gynecologists in many countries and the most common

  procedure is total abdominal hysterectomy (TAH). The incidence of laparoscopically assisted vaginal hysterectomy (LAVH) performed for benign lesions has progressively increased in recent years. Our objective was to compare the relative advantages and disadvantages of LAVH and TAH procedures.

  Methods: A clinical trial was performed on patients who were candidates for hysterectomy with benign reasons in Arash hospital from March 2006 to April 2007. By

  simple randomization, 90 patients (30 for LAVH and 60 for TAH) were selected. Demographic details and intra-operative and post-operative complications were recorded by the staff and were compared between the two groups.

  Results: On average, LAVH operations took significantly longer than TAH operations

  (100.17 ± 39.35 minutes 145.83 ± 41.55 minutes P< 0.0001). The total length of

  hospital stay was significantly shorter after LAVH than after TAH (3.43 ± 0.90 days

  3.94 ± 1.02 P= 0.025). Although the hemoglobin (gr/dl) drop in LAVH was significantly

  higher than TAH (1.22 ± 0.94 and 0.58 ± 0.82, P=0.0012), blood transfusions were more

  common in TAH (1 case versus 3 cases). The drug requirement to control pain during

  hospitalization after the two surgeries was not significantly different between the two

  groups. Fever was observed more often in the TAH group (P = 0.051). Finally, Intra-operative and post-operative complications were lower in LAVH than TAH.

  Conclusion: Although operation length is significantly higher in LAVH, this procedure

  is safer and more comfortable for patients and health care providers.


Afsaneh Tehranian, Bita ESLAMI,
Volume 22, Issue 4 (2-2009)


Hydatid disease is a widespread parasitic infection caused by tapeworm

Echinococcus and it affects mainly the liver, but other organs such as pelvic organs

could be involved very rare. Here we report a case of hydatid cyst with involvement

of oviduct in a woman with endometrial cancer. It was misdiagnosed as a multicystic

right ovarian mass before surgery and by microscopic study it was shown as a primary

degenerated hydatid cyst which was also unusual. Although pelvic echinococcal

cysts rarely occur, the gynecologists should be consider, and the possibility of a

hydatid cyst when they find a pelvic cystic mass, especially in areas where the disease

is endemic.

Zahra ESLAMI-Nejad, Noureddi Nematollahi-Mahanin, Fereshteh Saffari, Hamid Mollaii, S. Ali Mohammad Arabzadeh,
Volume 23, Issue 4 (2-2010)


  Background: The interest in using bacteria as anti- cancer therapeutic agents dates back to the end of the19th century. Some bacteria like Salmonella and Listeria replicate effectively inside malignant cell lines and suppress their growth. The bacterium Streptococcus pyogenes has become medically famous as a flesh-eating pathogen since mid-1980s. It is the causative agent of a life threatening clinical condition called necrotizing fasciitis. S. pyogenes usually produces a range of lytic enzymes that promote bacterial pathogenesis. With these characters, could this bacteria. be employed as a curing agent for certain cancers? The aim of this study was to determine the influence of S. pyogenes on malignant cellular death (apoptosis or necrosis)- in an ex-vivo "experimental- interventional" study.

  Methods: The cytotoxicity of fifteen internalized streptococcal strains( including 12

  clinical isolates, 2 known M types [M1, M3] and standard strain), on four types of malignant cell lines- A549, BT-20, PC-3, L-929- were tested by Trypan blue exclusion, DNAfragmentation and WST-1 methods. The streptococcal protease, lipase, DNase and serum opacity factor (SOF) were tested concurrently. The standard strain of Streptococcus (Enterococcus) faecalis was employed as negative control. The results were analyzed by statistical Minitab software.

  Results: The overall cytotoxicity rate of -internalized- S. pyogenes was 57% by trypan

  blue method and 50 % by DNA electrophoresis. False positive results occurred for the negative control in WST-1 therefore this test did not present reasonable results. The correlation between production of SOF, lipase, DNase and cytotoxicity of S. pyogenes was not significant (p > 0.05). However, 67% of the protease positive strains induced cellular death in at least one type of - malignant cell line (p<0.05).

  Conclusion: Our findings indicated that, some non-invasive S. pyogenes that cause benign infection like pharyngitis can induce cell death in various cancerous cell lines. It

  seemed that among bacterial products, the proteolytic enzymes- linked to the streptococcal pyrogenic exotoxin B (spe-B)- were more related to bacterial invasion.


Nasrin Faridi Tazeh-Kand, Ashraf Moini, Bita ESLAMI, Anooshe Khajehdehi,
Volume 25, Issue 4 (12-2011)


 Background and objective: Pain relief during labour is an important determinant of a women’s birth experience. There are numerous pain relief techniques which can be used either with or without pain medication. The aim of our study was to compare the effect of remifentanil alone and its effect in pain relief while using with ketamine during labour.

 Methods: After obtaining informed consent and approval of hospital ethics committee, 40 women with gestational age between 38 and 42 weeks gestation in early labour were recruited for this study. They were randomly allocated into two groups: group RK (20 cases) received 25 μg remifentanil as a starting dose and continuous infusion of 0.06 μg/kg/min remifentanil plus 0.5 mg/kg/h ketamine for 4 hours via pump and group R (20 cases) received 25 μg remifentanil as a starting dose and continous infusion of 0.06 μg/kg/min remifentanil.

 Results: The baseline of pain scores were similar in both groups (5.75 ± 2.51 vs 7 ± 2.45, p= 0.12) but after 30 minutes to 120 minutes the VAS scores were significantly higher in R group (p< 0.001). The rate of patients who were satisfied (excellent and very good) in RK was 80% but in R group was 45% (p = 0.03). Nausea and vomiting were significantly higher in R group (p<0.05).

 Conclusion: The remifentanil plus ketamine produced better pain relief during labour with continous monitoring than continous remifentanil with no adverse effects for mothers and infants.

Ali Sadegh, Nasrin Faridi Tazeh-Kand, Bita ESLAMI,
Volume 26, Issue 2 (5-2012)

 Background: Shivering is one of the common problems in spinal anesthesia. The objective of the present study was to evaluate the effect of intrathecal fentanyl (25 μg) on incidence and severity of intraoperative and postoperative shivering.


Methods: A double-blind randomized controlled study was conducted in eighty healthy women (ASA Physical status I) scheduled for elective cesarean section under spinal anesthesia. Subjects were randomly divided into two equal groups. The patients received 12.5 mg (2.5ml) of 0.5% hyperbaric bupivacaine combined with 25 μg (0.5 ml) fentanyl in Group F as a study group and 12.5 mg (2.5ml) of 0.5 % hyperbaric bupivacaine combined with 0.5 ml normal saline in Group S as a control group. Incidence of shivering during 30 and 60 minutes of surgery and recovery and complications were evaluated.

 Results: The total incidence of shivering in Group F was significantly lower than Group S (10% in group F 75% in group S, p< 0.0001). Almost all patients started shivering in the first hour after spinal anesthesia and the rate of shivering especially in second 30 minutes was higher than first 30 minutes in both groups. None in Group F but 22 patients (55%) in Group S had shivering during recovery and all of them reported shivering at the first 30 minute at recovery. The severity of shivering in Group F was significantly lower than Group S (p <0.0001).


Conclusion: Intrathecal bupivacaine combined with fentanyl is associated with a lower incidence and severity of shivering.

Omid Pournik, Sara Dorri, Hedieh Zabolinezhad, Seyyed Moayed Alavian, Saeid ESLAMI,
Volume 28, Issue 1 (1-2014)

  Background :Timely diagnosis of liver cirrhosis is vital for preventing further liver damage and giving the patient the chance of transplantation. Although biopsy of the liver is the gold standard for cirrhosis assessment, it has some risks and limitations and this has led to the development of new noninvasive methods to determine the stage and prognosis of the patients. We aimed to design an artificial neural network (ANN) model to diagnose cirrhosis patients with non-alcoholic fatty liver disease (NAFLD) using routine laboratory data.

  Methods : Data were collected from 392 patients with NAFLD by the Middle East Research Center in Tehran. Demographic variables, history of diabetes, INR, complete blood count, albumin, ALT, AST and other routine laboratory tests, examinations and medical history were gathered. Relevant variables were selected by means of feature extraction algorithm (Knime software) and were accredited by the experts. A neural network was developed using the MATLAB software.

  Results : The best obtained model was developed with two layers, eight neurons and TANSIG and PURLIN functions for layer one and output layer, respectively. The sensitivity and specificity of the model were 86.6% and 92.7%, respectively.

  Conclusion : The results of this study revealed that the neural network modeling may be able to provide a simple, noninvasive and accurate method for diagnosing cirrhosis only based on routine laboratory data.

Homa Rasoolijazi, Mehdi Mehdizadeh, Mansoureh Soleimani, Farnaz Nikbakhte, Mohsen ESLAMI Farsani, Shima Ababzadeh,
Volume 29, Issue 1 (1-2015)

  Background: The Rosemary extract (RE) possesses various antioxidant, cytoprotective and cognition-improving bioactivities. In this study, we postulated which doses of RE have a more effect on the hippocampus of middle-aged rats.

  Methods : In this experimental study, thirty-two middle-aged male Wistar rats were fed by different doses (50,100 and 200 mg/kg/day) of RE (containing 40% carnosic acid) or distilled water for 12 weeks. The effects of different RE doses on learning and spatial memory scores, hippocampal neuronal survival, antioxidant enzymes and lipid peroxidation amount were evaluated by one and two way analysis of variance (ANOVA).

  Results : It seemed that RE (100mg/kg) could recover the spatial memory retrieval score (p< 0.05). The amount of activity of SOD, GPx and CAT enzymes in the hippocampus of animals of the RE (100mg/kg) group showed a significant increase compared to the normal group (p< 0.01), (p< 0.01) and (p< 0.05), respectively. Also, the amount of activity of GPx in the RE (50 mg/kg) group of animals showed a significant increase compared to the normal group (p< 0.05). No significant difference was found between the groups in the MDA level.

  Conclusion : The results revealed that rosemary extract (40% carnosic acid) may improve the memory score and oxidative stress activity in middle aged rats in a dose dependent manner, especially in 100mg/kg.


Nader Roshan, Seyed Farshad Allameh, Omid ESLAMI,
Volume 32, Issue 1 (2-2018)

Ampullary neoplasms are one of the causes of obstructive jaundice. Endoscopic retrograde cholangiopancreatography (ERCP) is useful procedure for diagnosing and tissue sampling of ampullary neoplasms. Ampullectomy by resecting entire ampulla provides whole lesion for pathologic evaluation and also is appropriate for real pathologic staging for further management decision but ampullectomy considered as a heroic endoscopic procedure. We share our experience in this field and explain our results.

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